The Cell Image Library

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CIL:13543

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Helix Pomatia Lectin (HPL) (green) staining is redistributed out of the Golgi of HeLa cells after EGF treatment for 4 h, while the Golgi marker Giantin (red) is unaffected. HPL binds various glycans b...

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CIL:13544

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Helix Pomatia Lectin (HPL) (green) staining at the Golgi (Giantin) (red) in unstimulated HeLa cells. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-li...

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CIL:13545

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Anti-Tn staining (green) co-localizes with Helix Pomatia Lectin (HPL) (red) at the Golgi (Giantin) (gray) in unstimulated HeLa cells. The Tn antigen refers to terminal α-linked N-acetyl galactosamine...

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CIL:13546

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Anti-Tn staining (green) localizes away from the Golgi (Giantin) (gray) and into punctate cytoplasmic structures in HeLa cells after EGF treatment for 4h. Helix Pomatia Lectin (HPL) (red) staining is ...

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CIL:13547

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

GalNac-T1 staining (red) and Helix Pomatia Lectin (HPL) (green) colocalizes exclusively at the Golgi (Giantin) (gray) in unstimulated HeLa cells. The Tn antigen refers to terminal α-linked N-acetyl g...

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CIL:13548

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

After EGF treatment of HeLa cells for 4h, a significant amount of GalNac-T1 staining (red) is apparent in punctate and diffuse cytoplasmic cellular structures that stain positively for Helix Pomatia L...

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CIL:13549

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

In unstimulated HeLa cells, GalNac-T1 (gray) and Helix Pomatia Lectin (HPL) (green) colocalize exclusively at the Giantin-stained Golgi apparatus (red). The Tn antigen refers to terminal α-linked N-a...

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CIL:13550

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

After EGF treatment of HeLa cells for 4h, a significant amount of GalNac-T1 staining (gray) is apparent in punctate and diffuse cytoplasmic cellular structures that stain positively for Helix Pomatia ...

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CIL:13551

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Helix Pomatia Lectin (HPL) (green) redistribution out of the Golgi (Giantin) (red) in HeLa cells upon EGF stimulation for 4 h is inhibited by co-treatement of Src Kinase Inhibitor, SU6656. The Tn anti...

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CIL:13552

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Helix Pomatia Lectin (HPL) (green) colocalizes at the Golgi (Giantin) (red) in unstimulated HeLa cells. The image also shows GalNac-T1 staining (gray). The Tn antigen refers to terminal α-linked N-ac...

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CIL:13553

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Helix Pomatia Lectin (HPL) (green) is redistributed out of the Golgi (Giantin) (red) in HeLa cells upon EGF stimulation for 4h. The Tn antigen refers to terminal α-linked N-acetyl galactosamine resid...

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CIL:13554

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Helix Pomatia Lectin (HPL) (green) redistribution out of the Golgi (Giantin) (red) in HeLa cells upon EGF stimulation for 4 h is inhibited by co-treatement of Src Kinase Inhibitor, SKI. The Tn antigen...

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CIL:13555

NCBI Organism Classification: Homo sapiens
Biological Process: protein O-linked glycosylation via serine
Cellular Component: Golgi stack

GalNac-T1 (green) and ERGIC53 (ER-to-Golgi intermediate compartment) (red) colocalize in unstimulated HeLa cells moderately only at the Golgi apparatus. Helix Pomatia Lectin (HPL) (gray) staining is i...

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CIL:13556

NCBI Organism Classification: Homo sapiens
Biological Process: protein O-linked glycosylation via serine
Cellular Component: Golgi stack

GalNac-T1 (green) and ERGIC53 (ER-to-Golgi intermediate compartment) (red) colocalize in in novel punctate ER to Golgi Intermediate Compartment structures upon EGF treatment of HeLa cells for 4h. Hel...

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CIL:13557

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Helix Pomatia Lectin (HPL) (gray) staining is present exclusively at the Golgi (Giantin) (green) with no significant colocalization with ER marker, PDI (protein disulfide isomerase) (red) in unstimula...

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CIL:13558

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Helix Pomatia Lectin (HPL) (gray) staining is present in diffuse structures that stain with an ER marker, PDI (protein disulfide isomerase) (red) in HeLa cells upon EGF treatment for 4h. The image in...

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CIL:13559

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

An ER-trapped GalNac-T activity reporter (Muc-PTS; fused to GFP) (green) is targeted to the ER marked by PDI (protein disulfide isomerase) (red) in HeLa cells cotransfected with inactive Src (SrcK-mCh...

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CIL:13560

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

An ER-trapped GalNac-T activity reporter (Muc-PTS; fused to GFP) (green) is targeted to the ER and not the Golgi, marked by Giantin (red) in HeLa cells cotransfected with inactive Src (SrcK-mCherry, c...

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CIL:13561

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

An ER-trapped GalNac-T activity reporter (Muc-PTS; fused to GFP) (green) is targeted to the ER and not the Golgi, marked by Giantin (red) in HeLa cells cotransfected with active Src (SrcE-mCherry, con...

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CIL:13562

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

An ER-trapped GalNac-T activity reporter (Muc-PTS; fused to GFP) (green) is targeted to the ER marked by PDI (protein disulfide isomerase) (red) in HeLa cells cotransfected with active Src (SrcE-mCher...

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CIL:13563

NCBI Organism Classification: Homo sapiens
Biological Process: retrograde vesicle-mediated transport, Golgi to ER
Cellular Component: Golgi cisterna membrane

COP-I beta1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red), is redistributed out of the Golgi after EGF treatment of HeLa cells for 4 h. The increase in number and intensity of puncta...

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CIL:13564

NCBI Organism Classification: Homo sapiens
Biological Process: retrograde vesicle-mediated transport, Golgi to ER
Cellular Component: Golgi cisterna membrane

COP-I beta1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red) in unstimulated HeLa cells. Helix Pomatia Lectin (HPL) (gray) staining is also shown. HPL binds various glycans but the Tn a...

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CIL:13565

NCBI Organism Classification: Homo sapiens
Biological Process: retrograde vesicle-mediated transport, Golgi to ER
Cellular Component: Golgi cisterna membrane

COP-I gamma1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red) in unstimulated HeLa cells. Helix Pomatia Lectin (HPL) (gray) staining is also shown. HPL binds various glycans but the Tn ...

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CIL:13566

NCBI Organism Classification: Homo sapiens
Biological Process: retrograde vesicle-mediated transport, Golgi to ER
Cellular Component: Golgi cisterna membrane

COP-I gamma1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red), is redistributed out of the Golgi after EGF treatment of HeLa cells for 4 h. The increase in number and intensity of punct...

image is part of a group

image is suitable for quantitation

CIL:13567

NCBI Organism Classification: Homo sapiens
Biological Process: retrograde vesicle-mediated transport, Golgi to ER
Cellular Component: Golgi-associated vesicle

GalNac-T1 staining (green) colocalizes with COP-I beta1 (red) staining at the Golgi (HPL) (gray) in unstimulated HeLa cells. Helix Pomatia Lectin (HPL) binds various glycans but the Tn antigen in part...

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CIL:13568

NCBI Organism Classification: Homo sapiens
Biological Process: retrograde vesicle-mediated transport, Golgi to ER
Cellular Component: Golgi-associated vesicle

GalNac-T1 (green) is in dispersed punctate structures that stain positive for COP-I beta1 (red) after EGF treatment of HeLa cells for 4h. These data suggest that GalNAc-Ts are being redistributed from...

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CIL:13569

NCBI Organism Classification: Homo sapiens
Biological Process: retrograde vesicle-mediated transport, Golgi to ER
Cellular Component: Golgi-associated vesicle

GalNac-T1 staining (green) colocalizes with COP-I gamma1 (red) staining at the Golgi (HPL) (gray) in unstimulated HeLa cells. Helix Pomatia Lectin (HPL) binds various glycans but the Tn antigen in par...

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image is suitable for quantitation

CIL:13570

NCBI Organism Classification: Homo sapiens
Biological Process: retrograde vesicle-mediated transport, Golgi to ER
Cellular Component: Golgi-associated vesicle

GalNac-T1 (green) is in dispersed punctate structures that stain positive for COP-I gamma1 (red) after EGF treatment of HeLa cells for 4h. These data suggest that GalNAc-Ts are being redistributed fro...

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CIL:13571

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) colocalizes primarily at the Golgi (Giantin) (red) with Helix Pomatia Lectin (HPL) (green) in unstimulated HeLa cells. HPL binds various glycans but the Tn ant...

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CIL:13572

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Constitutively active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) colocalizes primarily at the Golgi (Giantin) (red) with Helix Pomatia Lectin (HPL) (green) in unstimulated HeLa cells. HPL binds various g...

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CIL:13573

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

After EGF stimulation for 4 h, Helix Pomatia Lectin (HPL) (green) in HeLa cells expressing wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) is redistributed from Golgi (Giantin) (red) to ER. HPL binds various...

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CIL:13574

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

After EGF stimulation for 4 h, Helix Pomatia Lectin (HPL) (green) in HeLa cells expressing constitutively active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) resists redistribution from Golgi (Giantin) (re...

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CIL:13575

NCBI Organism Classification: Mus musculus
Biological Process: signal transduction
Cellular Component: Golgi stack

Wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) does not block redistribution of Helix Pomatia Lectin (HPL) (green) from Golgi to ER in Src-deficient SYF fibroblasts cotransfected with active Src (SrcE-DsRe...

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CIL:13576

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Constituvely active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) blocks redistribution of Helix Pomatia Lectin (HPL) (green) from Golgi to ER in Src-deficient SYF fibroblasts cotransfected with active Src...

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CIL:13577

NCBI Organism Classification: Homo sapiens
Biological Process: Golgi organization
Cellular Component: Golgi stack

Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of Helix Pomatia Lectin (HPL) (green) from t...