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Helix Pomatia Lectin (HPL) (green) is redistributed out of the Golgi (Giantin) (red) in HeLa cells upon EGF stimulation for 4h. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HPL binds various glycans but the Tn antigen in particular. HeLa cells were serum starved overnight in DME (noFBS) and treated with human recombinant EGF (100 ng/ml; Sigma-Aldrich) and DMSO for 4h. Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100). Primary antibody staining followed the manufacturer's instructions. Cells were subsequently stained for 15â��30 min with secondary Alexa Fluorâ��conjugated antibodies (Alexa 488 for anti-Giantin). Hoechst (blue) and Alexa 647-conjugated-HPL were added during secondary antibody incubations. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with fluorescence excitation at 405 nm, 488 nm, and 633 nm and either a 60x objective (U Plan Super Apochromatic [UPLSAPO]; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 3A, top right panel, in J Cell Biol. 189: 843-858. 2010. Images in Fig 3 include CIL#s 13551, 13552, 13553, 13554.

Biological Sources

NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
cervical carcinoma
Cell Line
Cellular Component
Golgi stack
integral to membrane
Golgi cisterna membrane


David J. Gill
Joanne Chia
Jamie Senewiratne
Frederic Bard
J Cell Biol. 189: 843-858. 2010.


This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro


Spatial Axis Image Size Pixel Size
X 1024px .2070μm
Y 1024px .2070μm
Z 1 1.0μm
*CIL – Cell Image Library accession number. Please use this to reference an image.