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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9807*  Cite 
Description

A high resolution image and rare micrograph of the contractile vacuole and intact contractile vacuole membrane of Euplotes. Close examination at high magnification reveals the organization of microtubules lining the pore itself. The contractile vacuole is to the upper left on this micrograph; the exterior of the ciliate is to the lower right. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 7/29/67 by R. Allen with Philips 200 operating at 60kV. Neg. 9,000X. The raw negative was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Euplotes sp.
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Euplotes sp. from Carolina Biological Supply
Cellular Component
contractile vacuole pore
contractile vacuole
microtubule
contractile vacuole membranes
Biological Context
Biological Process
water homeostasis
ionic homeostasis
Molecular Function
structural constituent of cytoskeleton
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9807
Archival Resource Key (ARK)
ark:/b7295/w9cil9807
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4778px 1.7nm
Y 4887px 1.7nm