The Cell: An Image Library

Licensing

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CIL:9522^*

Description

The single chloroplast of the unicellular alga Chlamydomonas reinhardtii contains an orange carotenoid pigment spot that functions in phototaxis. The 'eyespot' is seen in this 250 nm epon section imaged with a JEM-1000 TEM at 1000KV as an array of dark spheres.

Technical Details

This image was taken at +6 degrees tilt - two others in the grouped image set were recorded at 0 and -6 degrees.

Biological Sources

NCBI Organism Classification: Chlamydomonas reinhardtii
Cell Type: plant cell
Cell Line: strain in culture
Cellular Component: chloroplast

Biological Context

Biological Process: photosynthesis, light harvesting; organelle organization

Attribution

Names: Mary Morphew; J. Richard McIntosh

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); film
Imaging Mode: FBbi:00000622; detection of electrons
Parameters Imaged: electron density
Source of Contrast: elastic scattering of electrons
Visualization Methods: uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2801px	0.8nm
Y	4510px	0.8nm

*CIL – Cell Image Library accession number. Please use this to reference an image.