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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9061*  Cite 
Description

The events of meiosis I and II in a living insect spermatocyte beginning with the breakdown of the nuclear envelope at diakinesis through to the completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 2 min over 5 hr 18 min by a QImaging Retiga EXi CCD camera. A movie prepared from this stack of images is included in the grouped set

Biological Sources
NCBI Organism Classification
Nephrotoma suturalis
Cell Type
spermatocyte
Cellular Component
cytoskeleton
spindle
nuclear chromosome
Biological Context
Biological Process
spindle assembly involved in male meiosis
Attribution
Names
Rudolf Oldenbourg
James R. LaFountain
Pubmed
15385630
Pubmed
12790452
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9061
Archival Resource Key (ARK)
ark:/b7295/w9cil9061
Grouping This image is part of a group.
Imaging
Image Type
recorded image
charge coupled device (CCD)
Processed image based on 5 recorded images
Image Mode
polarization microscopy
Parameters Imaged
UV/visible/IR photons
Source of Contrast
intrinsic birefringence
Visualization Methods
relative retardation (retardance) measurement
Processing History
5-frame algorithm
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 500px 87nm
Y 500px 87nm
Time 120 seconds 153