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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9058*  Cite 
Description

The events of meiosis II in a living insect spermatocyte beginning at the completion of prophase II through the comletion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 30 sec over 3 hr by a Dage-MTI C300 video camera with Scion AG-5 frame grabber. Raw images were processed using a 4-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).

Biological Sources
NCBI Organism Classification
Nephrotoma suturalis
Cell Type
spermatocyte
Cellular Component
cytoskeleton
spindle
nuclear chromosome
Biological Context
Biological Process
spindle assembly involved in male meiosis II
Attribution
Names
Rudolf Oldenbourg
James R. LaFountain
Pubmed
15385630
Pubmed
12790452
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9058
Archival Resource Key (ARK)
ark:/b7295/w9cil9058
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Processed image based on 5 recorded images
Image Mode
polarization microscopy
Parameters Imaged
UV/visible/IR photons
Source of Contrast
intrinsic birefringence
Visualization Methods
relative retardation (retardance) measurement
Processing History
4-frame algorithm
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 640px 87nm
Y 480px 87nm
Time 30 seconds 358