The Cell: An Image Library

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CIL:8952^*

Description

Microtubule dynamics in a U2OS (human osteosarcoma) cell. Cells were injected with Xrhodamine-tubulin and this time-lapse image series was collected on a spinning disk confocal microscope on a Nikon Ti200 using a 100X 1.4 NA Plan Apo objective with a 605x52 EM filter for 568 nm laser excitation. Images were collected with a Coolsnap HQ2 (Photometrics) with 700 ms exposure.

Biological Sources

NCBI Organism Classification: Homo sapiens; (human)
Cell Type: epithelial cell
Cell Line: U-2 OS
Cellular Component: microtubule

Biological Context

Biological Process: microtubule cytoskeleton organization
Molecular Function: microtubule plus-end binding

Attribution

Names: Yukako Nishimura; Clare M. Waterman
Other: Cell and Tissue Morphodynamics

Imaging

Image Type: recorded image
Imaging Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: X-Rhodamine
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1392px	0.105μm
Time	7 sec

*CIL – Cell Image Library accession number. Please use this to reference an image.