Licensing

Copyright
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Description

This time-lapse movie shows GFP-tagged ROD, a protein found at the condensed chromosome kinetochore, during the waves of synchronous mitoses. The sequence shows 1. kinetochore alignment at the cell equator in metaphase, 2. the splitting of sister chromosomes at anaphase onset and 3. kinetochore movement during anaphase until they reach the spindle poles in telophase. When the chromosomes decondense, the GFP-ROD signal disappears.

Technical Details

Time lapse 4D spinning disk confocal microscopy images were acquired every 8 seconds and are played at 6 frames/second. The images were acquired with a Nikon microscope (a X40 objective) and a Perkin-Elmer laser spinning disk. The program used to capture, pseudocolor, and process the images was Metamorph.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cellular Component
condensed chromosome kinetochore

Biological Context

Biological Process
mitosis
Molecular Function
detection of stimulus involved in mitotic cell cycle checkpoint

Attribution

Name
Rosalind Silverman-Gavrila

Imaging

Image Type
recorded image
Imaging Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
TagGFP
Data Qualifiers
processed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 250px ——
Y 510px ——
Time 6 sec
*CIL – Cell Image Library accession number. Please use this to reference an image.