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Human umbilical vein endothelial cells (HUVECS) labeled for focal adhesions (green) and F-actin (red). HUVECs were plated on polyacrilamide gels (1.5 Kpa) coated with 1.0 mg/ml fibronectin. After 24 hrs cells were fixed in 3% paraformaldehyde and stained with mouse antibody to Phosphotyrosine (4G10) monoclonal antibody detected with goat anti-mouse Alexa 565 and Alexa 488 phalloidin to stain for F-actin. Image was collected on a Nikon Eclipse with a cooled charge coupled device (CCD; Orca II; Hamamatsu Photonics) using a 60x 1.4 NA Plan Apo PH objective lens (Nikon). The exposure time was 320 msec each channel. A xenon arc lamp was used with a Semrock DA/FI/TR/Cy5-4x4M-B Sedat filter set.

Biological Sources

NCBI Organism Classification
Homo sapiens
Cell Type
primary cell line cell
endothelial cell
Cell Line
Cellular Component
focal adhesion
actin cytoskeleton

Biological Context

Biological Process
regulation of focal adhesion assembly
Molecular Function
phosphotyrosine binding


Ana M. Pasapera
Clare M. Waterman

Sample Preparation

formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue


Spatial Axis Image Size Pixel Size
X 1392px 0.107μm
Y 1040px 0.107μm
Channel Wavelength
1 488, 568 nm
*CIL – Cell Image Library accession number. Please use this to reference an image.