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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7438*  Cite 
Description

Human foreskin fibroblasts (HFF1) cells transfected with mKusabira-Orange EB3, a marker for growing microtubule ends. Note the dramatic movement of the bright fluorescent markers toward the cell periphery. Cells were imaged every 3 s by a spinning disk confocal microscoe on a Nikon Ti2000 using a 1.4NA Plan Apo Ex objective and a solid state 568 laser with a 605x52nm EM filter (Semrock). Images were recorded with a Coolsnap HQ2 (Photometrics) using 500 ms exposures.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
primary cell line cell
Cell Line
Prepuce of penis
human foreskin fibroblast (HFF)
Cellular Component
microtubule plus end
Biological Context
Biological Process
microtubule polymerization
Molecular Function
microtubule plus-end binding
Attribution
Names
Yukako Nishimura
Clare M. Waterman
OTHER
NHLBI/NIH
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7438
Archival Resource Key (ARK)
ark:/b7295/w9cil7438
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Kusabira OrangeFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1392px 0.105µm
Y 1040px 0.105µm
Time 3 seconds