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COS 7 cell expressing Organelle LightsTM PM-GFP (Invitrogen)consisting of the myristoylation/palmitoylation sequence from Lck tyrosine kinase. Images were collected with a 63X 1.4 NA Plan-Apochromat oil objective on a Zeiss LSM510 META (Carl Zeiss,Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter. Image size is 1024 x 1024, pixel size is 0.1 µm x 0.1 µm and pixel time was set to 1.6 µsec. Detector pinhole was set to collect 29 ~0.5 µm slices at 0.5 µm intervals

Biological Sources

NCBI Organism Classification
Chlorocebus sabaeus
(green monkey)
Cell Line
Cellular Component
plasma membrane

Biological Context

Biological Process
plasma membrane organization


George H. Patterson


Image Type
recorded image
Imaging Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
Visualization Methods
UV/visible/IR photons

Sample Preparation

living tissue
Relation To Intact Cell
living tissue


Spatial Axis Image Size Pixel Size
X 1024px 0.0952μm
Y 1024px 0.0952μm
Z 64 0.5μm
*CIL – Cell Image Library accession number. Please use this to reference an image.