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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:728*  Cite 
Description

COS 7 cell expressing Organelle LightsTM PM-GFP (Invitrogen)consisting of the myristoylation/palmitoylation sequence from Lck tyrosine kinase. Images were collected with a 63X 1.4 NA Plan-Apochromat oil objective on a Zeiss LSM510 META (Carl Zeiss,Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter. Image size is 1024 x 1024, pixel size is 0.1 µm x 0.1 µm and pixel time was set to 1.6 µsec. Detector pinhole was set to collect 29 ~0.5 µm slices at 0.5 µm intervals

Biological Sources
NCBI Organism Classification
Chlorocebus sabaeus
Cell Line
COS-7
Cellular Component
plasma membrane
Biological Context
Biological Process
plasma membrane organization
Attribution
Names
George H. Patterson
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL728
Archival Resource Key (ARK)
ark:/b7295/w9cil728
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
EGFP
Visualization Methods
UV/visible/IR photons
Sample Preparation
Methods
living tissue
Relation To Intact Cell
living tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.0952µm
Y 1024px 0.0952µm
Z 64px 0.5µm


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