Licensing

Pd
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Description

COS 7 cell expressing Organelle LightsTM PM-GFP (Invitrogen)consisting of the myristoylation/palmitoylation sequence from Lck tyrosine kinase. Images were collected with a 63X 1.4 NA Plan-Apochromat oil objective on a Zeiss LSM510 META (Carl Zeiss,Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter. Image size is 1024 x 1024, pixel size is 0.1 µm x 0.1 µm and pixel time was set to 1.6 µsec. Detector pinhole was set to collect 29 ~0.5 µm slices at 0.5 µm intervals

Biological Sources

NCBI Organism Classification
Chlorocebus sabaeus
(green monkey)
Cell Line
COS-7
Cellular Component
plasma membrane

Biological Context

Biological Process
plasma membrane organization

Attribution

Name
George H. Patterson

Imaging

Image Type
recorded image
Imaging Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
EGFP
Visualization Methods
UV/visible/IR photons

Sample Preparation

Methods
living tissue
Relation To Intact Cell
living tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 1024px 0.0952μm
Y 1024px 0.0952μm
Z 64 0.5μm
*CIL – Cell Image Library accession number. Please use this to reference an image.