Alternate header for print version

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:700*  Cite 

X-ray scattering shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contain more ordered chromatin than most nuclei. Here, fibers isolated from these nuclei have been negatively stained with aurothioglucose and imaged by TEM. ~30 nm chromatin fibers are seen, some exhibiting short regions with apparent structural order.

Technical Details

Chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films, negatively stained with aurothioglucose and observed at 80Kv using a CM10 TEM. See Woodcock CL et al. 1991. Ultrastructure of chromatin. I. Negative staining of isolated fibers. J Cell Sci. 99:99-106.

Biological Sources
NCBI Organism Classification
Necturus maculosus
Cell Type
nucleate erythrocyte
Cellular Component
nuclear chromatin
Biological Context
Biological Process
chromatin assembly
chromosome organization
Molecular Function
DNA packaging
Chris Woodcock
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
other negative stain
Processing History
raw unprocessed data
Sample Preparation
glutaraldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Spatial Axis Image Size Pixel Size
X 2334px 0.67nm
Y 1932px 0.67nm