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CIL:6518*  Cite 

This study of the zebrafish eye is a series of four images taken at increasing magnifications. The first image is the SEM block face of the zebrafish eye. This is followed by a second image that is a higher magnification TEM image of the plexiform layer and the outer nuclear layer of the zebrafish retina. This image, the third image in the series, is a 15,000X TEM image of the bipolar and horizontal cells interdigitating in the OPL layer of the zebrafish retina. The fourth image in the series is a higher magnification (60,000X) of the dense synaptic bar and synaptic vesicles. Bipolar and horizontal cells (H) interdigitate in the outer plixiform layer (OPL) of the zebrafish retina. The presynaptic cone membrane contains an arciform density (arrowhead) and synaptic vesicles (V). Image collected on a JOEL 1230 at 80 kV using a Gatan 967 slow-scan, cooled CCD camera at 15,000X. 70 nm thick section. Microwave processing, osmium tetroxoxide/osmid procedure for enhancing membranes, and copper lead en bloc staining. Procedure for specimen preparation available at

Biological Sources
NCBI Organism Classification
Danio rerio
Cell Type
Outer plexiform layer of retina
Bipolar cell of retina
Cell Line
Horizontal cell of retina
Cellular Component
Ribbon synapse
presynaptic membrane
Biological Context
Biological Process
retina layer formation
synaptic transmission
Zebrafish Anatomy
retinal outer plexiform layer
JoAnn Buchanan (Stanford)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
lead salt
copper salt
Processing History
unprocessed raw data
Spatial Axis Image Size Pixel Size
X 1200px 7nm
Y 1200px 7nm