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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:578*  Cite 
Description

Avian erythrocytes differ from most vertebrates in retaining the nucleus at maturity. During erythrocyte development, transcription is progressively shut down and nuclei become compact and heterochromatic. Since erythrocyte chromatin contains little other than histones and DNA, it is the material of choice for many in vivo and in vitro studies. This image, recorded on film at 100KV is a thin section, prepared from washed blood fixed with glutaraldehye, post-fixed with OsO4, embedded in plastic and stained with uranyl and lead salts. The dark, centrally located nucleus is prominent, and the chromatin so compact that the chromatin fiber organization is obscured.

Biological Sources
NCBI Organism Classification
Gallus gallus gallus
Cell Type
erythrocyte
Cellular Component
nuclear chromatin
Biological Context
Biological Process
aerobic respiration
nucleus organization
chromatin organization
Molecular Function
oxygen transport
Attribution
Names
Woodcock, Christopher
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL578
Archival Resource Key (ARK)
ark:/b7295/w9cil578
Grouping This image is part of a group.
Imaging
Image Type
recorded image
film
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
inelastic scattering
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Sample Preparation
Methods
glutaraldehyde fixed tissue
microtome-sectioned tissue
Relation To Intact Cell
tissue in plastic embedment
Dimensions
Spatial Axis Image Size Pixel Size
X 4148px 0.8nm
Y 2810px 0.8nm


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