NCBI Organism:Mus musculus; Cell Types:CNS neuron (sensu Vertebrata) Cell Components:dendritic spine, dendrite;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48401^* Cite

Description

Tomographic reconstruction of a dendrite and associated dendritic spines from a medium spiny neuron from the mouse nucleus accumens. This image is a maximum intensity projection of a segment of dendrite in a 2 um section. The cell was filled by intracellular injection of Lucifer Yellow dye and photooxidized. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.

Technical Details

The tomogram was generated using an JEOL4000 IVEM. Single tilt images spanned -60 to 60° in 2° increments. Magnification, 3000X; accelerating voltage, 400KeV.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: CNS neuron (sensu Vertebrata)
Cellular Component: dendritic spine; dendrite

Attribution

Names: Maryann Martone; Eric Bushong
Link: CCDB 1

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL48401
Archival Resource Key (ARK): ark:/b7295/w9cil48401

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: detection of electrons; illumination by electrons
Parameters Imaged: electron density
Data Qualifiers: processed data

Sample Preparation

Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1440px	0.119µm
Y	1782px	0.119µm
Z	233px	0.5µm