Licensing

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Description

Echinoderm sperm nuclei are unusual in that chromatin fibers are clearly seen, allowing their diameter to be accurately determined. Here, sperm from the starfish P. miniata were treated to remove the outer membrane, cryo-fixed by plunging into liquid ethane, freeze-substituted in acetone and low-temperature embedded in Lowicryl K11M. Thin sections were stained with uranyl acetate, and examined using a FEI CM10 TEM operated at 100KV. This image shows the central nucleus flanked by the crescent-shaped mitochondrion. The nucleus contains clearly-defined chromatin fibers, some of which are spilling out of the nuclear compartment.

Biological Sources

NCBI Organism Classification
Patiria miniata
Cell Type
sperm
Cellular Component
male germ cell nucleus

Biological Context

Biological Process
reproduction
nucleus organization
Molecular Function
structural constituent of chromatin

Attribution

Name
Chris Woodcock
Pubmed
8138565

Grouping

This image is representative of a group of similar images.

Imaging

Image Type
recorded image
film
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data

Sample Preparation

Methods
cryofixed tissue
freeze substitution
tissue in plastic embedment
Relation To Intact Cell
microtome-sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 2606px 1.1nm
Y 2216px 1.1nm
*CIL – Cell Image Library accession number. Please use this to reference an image.