Licensing

Attribution_by_nc_nd
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Description

The image shows a 3D-rendering (Imaris software) of a live confluent culture of NIH-3T3 cells obtained using confocal microscopy. The cells were co-transduced with 5 fluorescent proteins and with Lentiviral Gene Ontology (LeGO) vectors expressing Cerulean (blue), EGFP (green), Venus (yellow), tdTomato (magenta) or mCherry (red) fluorescent proteins to provide combinatorial colors for progeny tracking. Groups of nearby cells of the same color descended from same stem cells. This image is grouped with two others obtained using a similar imaging strategy. See also Malide et al. (2012) Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood. Dec 20;120(26):e105-16. An image obtained in the same manner that obtained an Honorable Mention in the 2011 Olympus BioScapes Digital Imaging Competition may be viewed as CIL:41631.

Technical Details

Imaging was performed using a Leica TCS SP5-AOBS 5-channel confocal system equipped with multiline Argon diode 561 nm, HeNe 594 nm and HeNe 633 nm. Images were recorded with a 40x 1.4 NA oil immersion objective lens, and 5 micrometer z-spacing and processed using Imaris software (Bitplane). See also: Malide D, Metais J-Y, and Dunbar CE. (2012). Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood. Dec 20;120(26):e105-16.

Biological Sources

NCBI Organism Classification
Mus musculus
(house mouse)
Cell Type
fibroblast
Cell Line
NIH/3T3
Cellular Component
cytoplasm

Biological Context

Biological Process
stem cell clonal tracking

Attribution

Names
Daniela Malide
Jean-Yves Metais
Cynthia Dunbar
Other
National Institutes of Health, Bethesda, MD

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
Visualization Methods
CeruleanFP
EGFP
VenusFP
tdTomatoFP
mCherryFP
Data Qualifiers
processed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 1104px ——
Y 1014px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.