Licensing

Pd
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
Comments: Powered by Pnyxe

Description

A time-lapse series using digital holographic microscopy presented as a movie. The movie shows living human prostate cancer cells (DU 145) induced to undergo of apoptosis following treatment with etoposide. The images were created by Phase Holographic Imaging AB (PHIAB), Lund, Sweden.

Technical Details

The human prostate cells were grown on Ibidi [Tm], 1-well µ-slides, (Ibidi GmbH, Martinsried, Germany) on a 37 °C heating plate (Ibidi). Images were recorded for over 61 hours with one image taken every 4 minutes. The video speed is 8 images per second and the images were artificially colored (Holostudio). The scale bar shows image size and the color bar represent specimen thickness. Contacts for additional details: Phase Holographic Imaging AB (PHIAB), Scheelevägen 22, 223 63 Lund, Sweden; Birgit Janicke Phone: +46 (0)46 38 60 80, +46 (0)07 5131681 Birgit.Janicke@phiab.se www.phiab.se

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
prostate cancer
Cell Line
DU145

Biological Context

Biological Process
execution phase of apoptosis

Attribution

Name
Birgit Janicke
Link
Phase Holographic Imaging

Imaging

Image Type
recorded image
Imaging Mode
digital Holographic microscopy
Parameters Imaged
phase shift of the laser light passing through the cell
Source of Contrast
phase contrast microscopy
Visualization Methods
Phase contrast holographic imaging
Processing History
Hstudio software (Phase Holographic Imaging AB)
Data Qualifiers
animation

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 1052px ——
Y 684px ——
Time 240 sec 915
*CIL – Cell Image Library accession number. Please use this to reference an image.