Licensing
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Description
Confocal image of a mitotic spindle in a dividing cell. The spindle is shown in yellow and the surrounding actin cytoskeleton is in blue. Images were acquired using a 100X NA 1.4 phase objective lens and a Perkin Elmer spinning disc confocal scan head. A Hammamatsu Orca ER cooled CCD camera was used to collect images. Image collection was driven using Metamorph software. Complete Z-series of images were collected and deconvolved using AutoQuant software. The image is a maximum intensity projection of a subset of the entire Z-stack; the same subset of the Z-stack is used in each channel. Sixth Prize, 2007 Olympus BioScapes Digital Imaging Competition.
Technical Details
Biological Sources
- NCBI Organism Classification
- Sus scrofa domestica
- (domestic pig)
- Cell Type
- epithelial cell
- Cell Line
- LLC-PK1
- Cellular Component
- spindle
- actin cytoskeleton
Biological Context
- Biological Process
- mitosis
Attribution
- Names
- Patricia Wadsworth
- 2007 Olympus BioScapes Digital Imaging Competition
- Link
- 2007 Olympus BioScapes Digital Imaging...
- Other
- University of Massachusetts Amherst, MA, USA
Imaging
- Image Type
- recorded image
- Imaging Mode
- confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of epitope
- Visualization Methods
- phalloidin
- primary antibody plus labeled secondary antibody
- Fluorescein (FITC)
- Rhodamine
- Processing History
- deconvolution
- maximum projection
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 1908px | —— |
| Y | 1620px | —— |
*CIL – Cell Image Library accession number. Please use this to reference an image.