The Cell: An Image Library - Image CIL:40593

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CIL:40593^*

Description

Colorized scanning electron micrograph of methicillin resistant staphylococcus aureus (MRSA) bacteria on the surface of a wound dressing.

Technical Details

Specimen was fixed with glutaraldehyde and dehydrated through an ethanol series. It was coated with 5nm gold-palladium and imaged at ultra high resolution (semi-immersion optics). The image was collected on a FEI Nova NanoSEM Family with the following imaging parameters: Magnification: 18,501 x, Horizontal Field Width: 16.1 microns, Vacuum: High vacuum (approx 10 e-06 Torr), Voltage: 5kV, Detector: TLD, Spot: 1.5 nA, and Working Distance: 4.3 mm

Biological Sources

NCBI Organism Classification: Staphylococcus aureus
Cellular Component: cell surface

Biological Context

Biological Process: antibiotic resistance

Attribution

Name: Paul Gunning
Link: FEI Life Sciences Images

Imaging

Image Type: recorded image
Imaging Mode: scanning electron microscopy (SEM)
Parameters Imaged: elastic scattering of electrons
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: visualization of contiguous regions; shadowing and plating; gold; palladium
Processing History: colorized

Sample Preparation

Methods: glutaraldehyde fixed tissue; ethanol dehydration

Dimensions

Spatial Axis	Image Size	Pixel Size
X	670px	——
Y	617px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.