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Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA022978 (shown in green). HPA022978 is a polyclonal anti-KRT24 antibody that labels intermediate filaments in these cells. Thee cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).

Technical Details

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Biological Sources

NCBI Organism Classification
Homo sapiens
Cell Type
Cell Line
U-2 OS
Cellular Component
intermediate filament
microtubule cytoskeleton

Biological Context

Biological Process
organelle organization
cytoskeleton organization


M Uhlen
P Oksvold
L Fagerberg
E Lundberg
K Jonasson
M Forsberg
M Zwahlen
C Kampf
K Wester
S Hober
H Wernerus
L Björling
F Ponten
Nat Biotechnol. 2010 28(12):1248-50.
Human Protein Atlas


This image is part of a group.


Image Type
recorded image
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data

Sample Preparation

chemically fixed tissue
permeabilized tissue
Relation To Intact Cell
dispersed cells in vitro


Spatial Axis Image Size Pixel Size
X 2048px ——
Y 2048px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.