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Transmission electron micrograph of a vacuole from a sertraline-treated sac1? mutant cell. The vacuolar membrane is moderately crenated. (25,000X). This image is part of a group of yeast vacuole images (CIL:40437-40448).

Technical Details

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources

NCBI Organism Classification
Saccharomyces cerevisiae
(Baker's Yeast, Brewer's Yeast)
Cell Type
sac1? mutant
Cellular Component

Biological Context

Biological Process
organelle organization
sertraline treatment


Ethan Perlstein
Daniel Korostyshevsky
Princeton EM Core Facility


This image is part of a group.


Image Type
recorded image
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data

Sample Preparation

glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
sectioned tissue


Spatial Axis Image Size Pixel Size
X 2700px ——
Y 2248px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.