Transmission electron micrograph of a representative sertraline-treated chc1 mutant cell. CHC1 encodes clathrin heavy chain, which trimerizes to form triskelia that coat vesicles. The vacuolar fragmentation phenotype is reversed by sertraline treatment. (8,000X). The untreated control image for the chc1 mutant is available as CIL 40416.
This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.