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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40400*  Cite 

Stack of time series images showing the change in microtubule distribution in cultured porcine kidney epithelial cells (LLCPK1) stably expressing GFP-alpha tubulin during the progression from prophase to prometaphase. The cytoskeleton is reorganized with disassembly of individual microtubules and formation of microtubule bundles, causing an inward collapse of the microtubule array prior to the formation of the mitotic spindle. A movie created from the stack is grouped with the image.

Technical Details

Living LLCPK1 cells expressing GFP-alpha-tubulin were observed using a Nikon Eclipse TE 300 microscope equipped with a 100x phase NA 1.4 objective lens, and images captured at 2 sec intervals with a Perkin Elmer spinning disc confocal scan head and a cooled CCD camera See also NM Rusan et al. 2002 Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport. J Cell Biol 158:997-1003.

Biological Sources
NCBI Organism Classification
Sus scrofa domestica
Cell Type
kidney epithelial cell
Cell Line
Cellular Component
microtubule cytoskeleton
Biological Context
Biological Process
microtubule cytoskeleton organization
Nasser Rusan
Patricia Wadsworth
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
charge coupled device (CCD)
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Data Qualifiers
processed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 650px ——
Y 515px ——
Time 2 seconds 253