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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40115*  Cite 
Description

Electron micrograph of cultured Drosophila Dl1 cells infected with flock house virus, prepared using chemical fixation and high pressure freezing followed by freeze substitution. This cell was prepared as part of an experiment to investigate different protocols for high pressure freezing. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Fixation: The media was removed and saved. Then, cell pellets were fixed in 2% glutaraldehyde in 100 mM cacodylate buffer for 30 minutes on ice. The fixed pellet was resuspended in media and centrifuged again. Cells were loaded into the 100 mm well of a type A brass planchette (Ted Pella, Inc. Redding, CA) and fast frozen in the Bal-Tec HPM010 (Bal-Tec, Liechtenstein). Freeze substitution: After freezing, samples (2) and (3) were placed into a Leica EM AFS Freeze substitution (FS) machine (Leica Microsystems, Bannockburn, IL) and incubated at -90 deg C for 24 hours in 0.1 percent tannic acid in acetone. Samples were washed three times with cold acetone (cooled to -90 degrees C) over 5 minutes, and placed in 1 percent OsO4 and 0.1% UA in cold acetone for 72 hours and held at -90 degrees C. After slowly warming to room temperature at 5 degrees C per hour, the specimens were rinsed in pure acetone three times (10 min. at room temperature). Infiltration and embedding in Durcupan resin was subsequently performed at room temperature.Images were acquired using a JEOL4000EX IVEM, magnification: 30000.0; accelerating voltage: 80.0 keV.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
embryonic cell
Cellular Component
cell body
Attribution
Names
Mark Ellisman
Gina Sosinsky
Ying Jones
Link
CCDB 3939
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40115
Archival Resource Key (ARK)
ark:/b7295/w9cil40115
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
Parameters Imaged
electron density
Data Qualifiers
processed data
Sample Preparation
Methods
osmium tetroxide fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——


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