Reconstruction


Reconstruction Data Files

Image, 512x512 image, Purkinje neuron injected with Lucifer Yellow.
Download (1MB)

Animation video, Rotation loop of a maximum intensity projection of a Purkinje neuron injected with Lucifer Yellow, rotated along the y axis
Download (12.25MB)

Reconstruction Data Files,
Download (49.0MB)


Segmentation


Segmentation Data Files

Image, 512x512 image, neurolucida
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Segmentation Data Files,
Download (569.92KB)

Licensing

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Description

A Purkinje neuron from the cerebellum of a 2 month old male C57BL/6 mouse, injected with Lucifer Yellow. The elaborate, highly branched dendritic arbor is revealed in this maximum projection image which was generated by compiling a z-series of optical sections. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Tissue was perfused with paraformaldehyde, sectioned (100 µm) on a vibratome and mounted in gelvatol, and imaged on a Biorad Radiance 2000 Confocal using a 60x 1.4NA objective.

Biological Sources

NCBI Organism Classification
Mus musculus
(house mouse)
Cell Type
Purkinje cell
Cellular Component
dendrite
neuronal cell body

Biological Context

Biological Process
cell projection organization

Attribution

Names
Maryann Martone
Diana Price
Andrea Thor
Masako Terada
Hiro Hakozaki
Link
CCDB 16
Project
P1170
See other images in this project

Grouping

This image is representative of a group of similar images.

Imaging

Image Type
recorded image
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
intracellular filling
Visualization Methods
lucifer yellow
Data Qualifiers
processed data

Sample Preparation

Relation To Intact Cell
vibratome-sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 512px ——
Y 533px ——

Detailed Project Information                           hide

Project ID: P1170
Leader: Maryann Martone
Collaborators: Diana Price, Andrea Thor, Masako Terada, Hiro Hakozaki
Description: Neurolucida tracing of filled Purkinje neurons
Experimental purpose: To obtain multi resolution data for Mouse BIRN
Species: mouse
System: central nervous system
Organ: brain
Region: cerebellum
Cell type: Purkinje neuron
Structure: dendritic tree
Product type: confocal
Instrument: Biorad Radiance 2000 Confocal
Start/End Dates: 2/5/2002 - unknown

Specimen Preparation                                     hide

Microscopy Product ID: 16
Image Basename: e4cb2a2
Protocol Description: NA

Detailed Imaging Parameters                          hide

Light microscopy product
Coverslip thickness: 1.0 um
Lens magnification: 40.0 X
Numerical aperture: 1.3
Immersion medium: oil
Mounting medium: gelvatol

Imaging Product Types                                    hide

Optical Section
Optical Z resolution: 0.25 um
Cutting plane: transverse


*CIL – Cell Image Library accession number. Please use this to reference an image.