Licensing

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Description

Scanning electron microscope image of Nicotiana alata stem cross section. Image shows outer epidermal layer, followed by the cortex and then large vascular bundles. The vascular bundles contain the phloem (nearest the cortex) and xylem.

Technical Details

See http://remf.dartmouth.edu:8080/EM-Wiki/36 for technical protocol

Biological Sources

NCBI Organism Classification
Nicotiana alata
(jasmine tobacco)

Biological Context

Biological Process
plant stem organization
Plant Growth
epidermal layer
stem cortex
phloem
xylem

Attribution

Name
Louisa Howard
Link
Dartmouth College EM facility

Imaging

Image Type
recorded image
Imaging Mode
scanning electron microscopy (SEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
uranyl salt
shadowing and plating
Processing History
unprocessed raw data

Sample Preparation

Methods
glutaraldehyde fixed tissue
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 4000px ——
Y 4000px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.