Freeze-fracture image of a barley (Hordeum vulgare) chloroplast from a mutant lacking chlorophyll b illustrating the changes from wildtype in particle distribution on the four types of cleaved thylakoid membrane face (PFs, PFu, EFs and EFu). See
Wildtype and mutant plants were were grown under a 16-hr day regime, harvested at 3 weeks, and chloroplasts isolated. For freeze fracture, isolated chloroplasts were made 30% in glycerol, plunge frozen, and cleaved, etched, and shadowed in a Balzers freeze-fracture unit. Replicas were examined with a Philips 300 transmission EM and images recorded on film. See Fig 3 in KR Miller et al. 1976 J. Cell Biol 71, 624-638.
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