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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38868*  Cite 
Description

A grazing section through the dorsal surface of Euplotes showing cross sections of cilia in four of the sparsely ciliated kineties . A layer of mitochondria underlies the pellicle. A replication band of the DNA in the macronucleus is typical of hypotrichs. The basal bodies and cilia of one cirrus are found as well as the contractile vacuole. EM taken on 8/1/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,800X. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Euplotes sp.
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Euplotes sp. from Carolina Biological Supply
Cellular Component
cilium
cytoplasm
macronucleus
contractile vacuole
Biological Context
Biological Process
ciliary or flagellar motility
macronucleus organization
DNA replication checkpoint
cytoplasm organization
Molecular Function
visual nuclear replication
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38868
Archival Resource Key (ARK)
ark:/b7295/w9cil38868
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4000px 2.8nm
Y 3028px 2.8nm


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