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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38860*  Cite 
Description

Contractile vacuole in the opisthe of a dividing cell. This is presumably the mature CV of the original “mother” cell. Spongiome tubules form large regions of additional membrane on two sides of the CV. The CV contains membrane-like layers that may be lipid. A food vacuole is observed containing expanded trichocysts of Paramecium. TEM taken on 5/20/69 by R. Allen with Philips 300 operating at 60kV. Neg. 5,000X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Didinium nasutum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Company, NC, U.S.A.
Cellular Component
contractile vacuole
smooth endoplasmic reticulum
extrusomes
Biological Context
Biological Process
contractile vacuole organization
smooth endoplasmic reticulum organization
Molecular Function
structural constituent of contractile vacuole
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38860
Archival Resource Key (ARK)
ark:/b7295/w9cil38860
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4000px 1nm
Y 2560px 1nm


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