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Description

This sequence taken with the polarizing microscope in 1967 shows the reversible enhancement of spindle volume and birefringence by heavy water in arrested metaphase oocyte of Pectinaria gouldi (ice cream cone worm). In heavy water (45% D2O, middle panel) the spindles get much larger as more tubulin is incorporated into microtubules. This work was conducted before the discovery of how to assemble microtubules in vitro in the early 1970s.

Technical Details

See: S. Inoue and S. Sato (1067) Cell motility by labile association of molecules. J Gen Physiol 50:259-292

Biological Sources

NCBI Organism Classification
Pectinaria gouldii
(Ice cream cone worm)
Cell Type
oocyte
Cellular Component
spindle

Biological Context

Biological Process
mitosis

Attribution

Names
S. Inoue
S. Sato
Publisheds
S. Inoue and S. Sato (1067) Cell motility by labile association of molecules. J Gen Physiol 50:259-292
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA

Imaging

Image Type
recorded image
Imaging Mode
polarization microscopy
Parameters Imaged
retardance
Source of Contrast
form birefringence
Visualization Methods
visualization of contiguous regions
Processing History
montage
Data Qualifiers
processed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 4068px ——
Y 2196px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.