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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38677*  Cite 
Description

This sequence taken with the polarizing microscope in 1967 shows the reversible enhancement of spindle volume and birefringence by heavy water in arrested metaphase oocyte of Pectinaria gouldi (ice cream cone worm). In heavy water (45% D2O, middle panel) the spindles get much larger as more tubulin is incorporated into microtubules. This work was conducted before the discovery of how to assemble microtubules in vitro in the early 1970s.

Technical Details

See: S. Inoue and S. Sato (1067) Cell motility by labile association of molecules. J Gen Physiol 50:259-292

Biological Sources
NCBI Organism Classification
Pectinaria gouldii
Cell Type
oocyte
Cellular Component
spindle
Biological Context
Biological Process
mitosis
Attribution
Names
S. Inoue
S. Sato
Published
S. Inoue and S. Sato (1067) Cell motility by labile association of molecules. J Gen Physiol 50:259-292
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38677
Archival Resource Key (ARK)
ark:/b7295/w9cil38677
Imaging
Image Type
recorded image
Image Mode
polarization microscopy
Parameters Imaged
retardance
Source of Contrast
form birefringence
Visualization Methods
visualization of contiguous regions
Processing History
montage
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 4068px ——
Y 2196px ——


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