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Description

Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug latrunculin A (disrupts the actin cytoskeleton) at frame 13. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations.

Technical Details

C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with latrunculin A. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video2 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.

Biological Sources

NCBI Organism Classification
Caenorhabditis elegans
(Nematode)
Cell Type
early embryonic cell
Cellular Component
cell cortex

Attribution

Names
Nathan W. Goehring
Carsten Hoege
Stephan W. Grill
Anthony A. Hyman
Published
J Cell Biol. 2011. 193(3): 583-594.
Link
http://jcb-dataviewer.rupress.org/jcb/...
Pubmed
21518794

Grouping

This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 354px .2050μm
Y 345px .2050μm
Time 30 sec 80
*CIL – Cell Image Library accession number. Please use this to reference an image.