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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37933*  Cite 
Description

Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug cytochalasin D (disrupts the actin cytoskeleton) at frame 19. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations.

Technical Details

C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video1 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.

Biological Sources
NCBI Organism Classification
Caenorhabditis elegans
Cell Type
early embryonic cell
Cellular Component
cell cortex
Attribution
Names
Nathan W. Goehring
Carsten Hoege
Stephan W. Grill
Anthony A. Hyman
Published
J Cell Biol. 2011. 193(3): 583-594.
Pubmed
21518794
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL37933
Archival Resource Key (ARK)
ark:/b7295/w9cil37933
Grouping This image is part of a group.
Imaging
Image Type
recorded image
time lapse microscopy
Image Mode
widefield illumination
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
compartmentalization of stain or label
Visualization Methods
mCherryFP
EGFP
Hoechst
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 382px 0.205µm
Y 360px 0.205µm
Time 30 seconds 50


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