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Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug cytochalasin D (disrupts the actin cytoskeleton) at frame 19. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations.

Technical Details

C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video1 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594.

Biological Sources

NCBI Organism Classification
Caenorhabditis elegans
Cell Type
early embryonic cell
Cellular Component
cell cortex


Nathan W. Goehring
Carsten Hoege
Stephan W. Grill
Anthony A. Hyman
J Cell Biol. 2011. 193(3): 583-594.


This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

living tissue
Relation To Intact Cell
whole mounted tissue


Spatial Axis Image Size Pixel Size
X 382px .2050μm
Y 360px .2050μm
Time 30 sec 50
*CIL – Cell Image Library accession number. Please use this to reference an image.