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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37252*  Cite 
Description

An electron micrograph showing a central longitudinal section of the unicellular green alga Chlamydomonas reinhardtii (y-1 yellow mutant) grown in the light. The single cup shaped chloroplast with pyrenoid (storage granule) surrounds the central nucleus and other organelles. Osmiophilic globules and a few starch granules are scattered among the thylakoid membranes. The pyrenoid appears as a large, finely granular mass of polygonal profile surrounded by discontinuous shell of starch plates and penetrated by a system of tubules. Surrounded by the chloroplast are the nucleus, dictyosomes (Golgi bodies) and their associated vacuoles, and endoplasmic reticulum cisternae of transitional type. Mitochondria are concentrated at the anterior pole of the cell and between the chloroplast and the cell membrane. A contractile vacuole and a flagellum are also visible.

Technical Details

Image corresponds to figure 2 in J Cell Biol. 1967 Dec;35(3):521-52. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification 8,400.

Biological Sources
NCBI Organism Classification
Chlamydomonas reinhardtii
Cellular Component
chloroplast
nucleus
flagellum
endoplasmic reticulum
Golgi stack
mitochondrion
pyrenoid
Biological Context
Biological Process
organelle organization
Attribution
Names
I. Ohad
Published
J Cell Biol. 1967 Dec;35(3):521-52.
Pubmed
6064364
Link
George E. Palade EM Slide Collection
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL37252
Archival Resource Key (ARK)
ark:/b7295/w9cil37252
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
Methods
osmium tetroxide fixed tissue
tissue in plastic embedment
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 6000px ——
Y 4694px ——