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Description

Electron microscope autoradiograph showing the high concentration of radioactivity over the condensing vacuoles of the Golgi complex twenty minutes after injection. Image corresponds to Figure 7 in J. Cell Biol. 20:473-495. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Anesthetized animals were intravenously injected with 1-5 millicuris of DL-leucine 4,5-H^3 with a specific activity of 3570 mc/mM. At times ranging from 4 min to 15 hrs after injection the pancreas was removed and fixed in osmium tetroxide. Tissue was embedded in methacrylate, sectioned, and post-stained with uranyl acetate. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x30,000.

Biological Sources

NCBI Organism Classification
Cavia porcellus
(Domesticated Guinea Pig)
Cell Type
pancreas cell
Cellular Component
vacuole
Golgi-associated vesicle

Biological Context

Biological Process
protein secretion
protein transport

Attribution

Name
Lucien Caro
Published
J Cell Biol. 1964 Mar;20:473-95.
Link
George E. Palade EM Slide Collection
Pubmed
14128049
Date
06/04/1964

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
transmission electron microscopy (TEM)
electron microscope autoradiograph
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
metabolically incorporated radioisotope
Processing History
unprocessed raw data

Sample Preparation

Methods
osmium tetroxide fixed tissue
Relation To Intact Cell
sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 6000px ——
Y 5094px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.