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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37119*
Description

Electron microscope autoradiograph showing the high concentration of radioactivity over the condensing vacuoles of the Golgi complex twenty minutes after injection. Image corresponds to Figure 7 in J. Cell Biol. 20:473-495. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

Technical Details

Anesthetized animals were intravenously injected with 1-5 millicuris of DL-leucine 4,5-H^3 with a specific activity of 3570 mc/mM. At times ranging from 4 min to 15 hrs after injection the pancreas was removed and fixed in osmium tetroxide. Tissue was embedded in methacrylate, sectioned, and post-stained with uranyl acetate. Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original Magnification: x30,000.

Biological Sources
NCBI Organism Classification
Cavia porcellus
Cell Type
pancreas cell
Cellular Component
vacuole
Golgi-associated vesicle
Biological Context
Biological Process
protein secretion
protein transport
Attribution
Names
Lucien Caro
Published
J Cell Biol. 1964 Mar;20:473-95.
Pubmed
14128049
Link
George E. Palade EM Slide Collection
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
electron microscope autoradiograph
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
metabolically incorporated radioisotope
Processing History
unprocessed raw data
Sample Preparation
Methods
osmium tetroxide fixed tissue
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 6000px ——
Y 5094px ——


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