Licensing

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Description

Nucleoli in a macronucleus of a cell undergoing micronuclear division. The nucleolus appears to have an organized surface layer composed of parallel threads of subunits. These may be developing ribosomal subunits that will soon be transported to the cytoplasm through the nuclear pores. TEM taken on 3/30/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 15,759X. Bar = 0.2µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39205) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
macronucleus
nuclear pore
nucleolus

Biological Context

Biological Process
macronucleus organization

Attribution

Name
Richard Allen (University of Hawaii)
Link
http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type
transmission electron microscopy (TEM)
illumination by electrons
Imaging Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data

Dimensions

Spatial Axis Image Size Pixel Size
X 3054px ——
Y 3261px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.