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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36747*  Cite 

A freeze-fracture image of a DV-II transforming into a DV-III by rapid fusion of lysosomes with its membrane. The P-face shown here of the early DV-III has abundant IMPs as well as many inward blebs (many are fractured off) and a wrinkled surface topography indicating recent lysosome fusion with the membrane. Some lysosomes still lie around the DV as well as many smaller vesicles. Blebs however only extend inward toward the lumen rather than into the cytosol. TEM taken on 9/27/76 by R. Allen with JEM-100B operating at 80kV. Neg. 9,719X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
food vacuole
Biological Context
Biological Process
digestive system process
Richard Allen (University of Hawaii)
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
shadowing and plating
recorded image
Print of negative scanned for Photoshop
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2118px ——
Y 2598px ——