The Cell: An Image Library - Image CIL:35288

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CIL:35288^*

Description

Living Lilium pollen tube labeled with dichloro-flourescein diacetate to mark the vacuole and imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice.The growing tip is devoid of vacuole. Other images in the group reveal the distributions of additional cytoplasmic components.

Technical Details

Living lily pollen tubes were labeled and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Image shows a central x-y plane through the tube. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.

Biological Sources

NCBI Organism Classification: Lilium formosanum; (St Joseph's lily)
Cell Type: pollen tube
Cellular Component: plant-type vacuole lumen

Biological Context

Biological Process: pollen tube growth

Attribution

Names: Alenka Lovy-Wheeler; Luis Cardenas; Joseph Kunkel; Peter K Hepler

Grouping

This image is part of a group.

Imaging

Image Type: photomultiplier tube (PMT)
Imaging Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: compartmentalization of stain or label
Visualization Methods: carboxy-dichloroflourescein diacetate
Processing History: central x-y slice

Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	500px	——
Y	178px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.