Constitutive expression of GFP (Green Fluorescent protein) in MTLn3 cells, a metastatic rat breast carcinoma line. This time lapse sequence of a single optical section near the substrate shows the cells jostling and the intrinsic movement of organelles that appear dark because they do not contain the fluorescent protein. This cell line is useful both as a cell culture system and also as a source of fluorescent cells that may be used to study metastasis in live rats or mice.
BioRad Radiance 2000 laser scanning confocal microscope with Kr/Ar laser for excitation of permanently transfected GFP at 488 nm. The pixel size is approximate.
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