Time lapse movie of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using DIC optics. This movie and the stack of images used to prepare it are grouped in the Cell Image Library.
Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 100x HCX PLAN APO NA 1.35 objective lens. images were captured at 30 sec intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 188.8.131.52 software.
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