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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34524*  Cite 
Description

Human sperm diluted in buffer imaged at room temperature within two hours of ejaculation. Cell at center swims at constant speed.

Technical Details

Sperm were diluted with PBS and plated in a coverslip bottom chamber on an Olympus IX70 with 60X N.A. 1.4 phase 3 optics. Images were collected with an air cooled CCD camera (either Roper Coolsnap or Cooke Sensicam) at 12 bits and converted to 8 bits. The time interval was less than 1 second per image.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
sperm
Cell Line
Mature sperm cell
Cellular Component
flagellum
Biological Context
Biological Process
ciliary or flagellar motility
sperm motility
Human Development Anatomy
gonadal component
Attribution
Names
Michael Crammer
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL34524
Archival Resource Key (ARK)
ark:/b7295/w9cil34524
Imaging
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
living tissue
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 640px 0.22µm
Y 480px 0.22µm


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