Human sperm diluted in buffer imaged at room temperature within two hours of ejaculation. Cell at center swims at constant speed.
Sperm were diluted with PBS and plated in a coverslip bottom chamber on an Olympus IX70 with 60X N.A. 1.4 phase 3 optics. Images were collected with an air cooled CCD camera (either Roper Coolsnap or Cooke Sensicam) at 12 bits and converted to 8 bits. The time interval was less than 1 second per image.