Licensing

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Description

Human sperm diluted in buffer imaged at room temperature within two hours of ejaculation. Cell at center swims at constant speed.

Technical Details

Sperm were diluted with PBS and plated in a coverslip bottom chamber on an Olympus IX70 with 60X N.A. 1.4 phase 3 optics. Images were collected with an air cooled CCD camera (either Roper Coolsnap or Cooke Sensicam) at 12 bits and converted to 8 bits. The time interval was less than 1 second per image.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
sperm
Cell Line
Mature sperm cell
Cellular Component
flagellum

Biological Context

Biological Process
ciliary or flagellar motility
sperm motility
Human Development Anatomy
gonadal component

Attribution

Name
Michael Crammer
Date
07/2004

Imaging

Image Type
recorded image
Imaging Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
living tissue
Data Qualifiers
processed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 640px 0.22μm
Y 480px 0.22μm
Time -- sec 140
*CIL – Cell Image Library accession number. Please use this to reference an image.