Licensing

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Description

This image set is of Transfluor assay where an orphan GPCR (G-protein coupled receptor) is stably integrated into a beta-arrestin GFP (green) expressing U2OS cell line also showing nucleus (red). After one hour incubation with a compound, the cells were quenched with fixative (formaldehyde) and the plate was read on Cellomics ArrayScan HCS Reader using the GPCR Bioapplication. Negative results are a more uniform distribution of GFP throughout the cytoplasm and positive results are a peri-nuclear localization. Recommended attribution: "We used the SBS Roche Transfluor image set provided by Ilya Ravkin and available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)." Analysis of the data set can be downloaded from http://www.broadinstitute.org/bbbc/sbs_roche_transfluor.html

Technical Details

The images constitute one row of a 348 well plate. The dose curve consists of 11 dose points and one control. Each concentration is duplicated in adjacent wells. Each well has three fields.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
osteocarcenoma
Cell Line
U-2 OS
Cellular Component
nucleus
beta arrestin

Biological Context

Biological Process
cellular localization

Attribution

Name
Ilya Ravkin
Link
http://www.broadinstitute.org/bbbc/sbs...

Grouping

This image is representative of a group of similar images.

Imaging

Image Type
recorded image
Imaging Mode
plate reader
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
distribution of DNA
Visualization Methods
EGFP
Processing History
unprocessed raw data

Sample Preparation

Methods
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.