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Description

A confocal Z-stack from a transgenic Drosophila expressing the kinesin-5 motor KLP61F-GFP embryo during prometaphase phase 1. KOP61-F is required to prevent prometaphase spindle collapse driven by the kinesin-14 Ncd. This image is original data contributing to Fig. 1 "Prometaphase spindle dynamics in Drosophila embryos: steady-state and elongation phase" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.

Technical Details

Embryos expressing GFP-KLP61F were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). For these experiments, z-stacks were acquired with an Orca II CCD camera (Hamamatsu Photonics) at intervals of 0.1-0.2 µm, using exposure times of 300-600ms. For further information see J. Cell Biol. 188(1):49-68.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cell Type
early embryonic cell
Cellular Component
spindle microtubule
kinesin complex

Attribution

Names
Gul Civelekoglu-Scholey
Li Tao
Ingrid Brust-Mascher
Roy Wollman
Jonathan M. Scholey
Published
J Cell Biol. 2010 Jan 11;188(1):49-68.
Link
jcb-dataviewer
Pubmed
20065089

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Green fluorescent proteins from Aequorea
Data Qualifiers
raw, unprocessed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 672px ——
Y 512px ——
Z 62 ——
*CIL – Cell Image Library accession number. Please use this to reference an image.