Licensing

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Description

HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein Snu114 and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The Snu114 is confined to the nucleoplasm. From Fig 1A in Huranova et al. 2010 J Cell Biol 191:75-86.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
cervical epithelium
Cell Line
HeLa
Cellular Component
pre-mRNA
snRNP
spliceosomal complex

Biological Context

Biological Process
RNA splicing
RNA metabolic process

Attribution

Names
M. Huranova
I. Ivani
A. Benda
I. Poser
Y. Brody
M. Hof
Y. Shav-Tal
K.M. Neugebauer
D. Stanek
Published
M Huranova et al. 2010 J Cell Biol 191:75-86

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
frame averaging
Data Qualifiers
processed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.