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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25778*  Cite 
Description

Elongation of the dorsal trunk ectoderm closely matches that of the ventral body side, but shows very little convergence. This time-lapse movie shows the elongation of the dorsal region of Platynereis between 54 and 58.66 hours post fertilization at 26C. The movie covers a period of 280 min in 56 frames. This is Movie 3 in supporting information for Proc Natl Acad Sci (2007) 104: 2727-2732.

Technical Details

To label cellular outlines, embryos were incubated for 15 min in 5 μM BODIPY564/570 coupled to propionic acid (Invitrogen, Carlsbad, CA) in natural seawater, then rinsed twice in natural seawater and mounted in a 1:1 mixture of natural seawater and 7.5% MgCl2 to prevent muscular contractions. Time-lapse recordings were performed with a PerkinElmer (Wellesley, MA) Ultraview RS System (spinning disk confocal). Embryos were kept during recordings (12 frames per hour) at a constant temperature of 25C or 26C in natural seawater between slide and coverslip separated by two layers of adhesive tape and silicone paste. The chamber was sealed with mineral oil (Sigma, St. Louis, MO).

Biological Sources
NCBI Organism Classification
Platynereis dumerilii
Cell Type
neurecto-epithelial cell
Biological Context
Biological Process
morphogenesis of an epithelium
axis elongation
Attribution
Names
Patrick R. H. Steinmetz
Fabiola Zelada-Gonzáles
Carola Burgtorf
Joachim Wittbrodt
Detlev Arendt
Published
Proc Natl Acad Sci (2007) 104: 2727-2732.
Pubmed
17301244
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25778
Archival Resource Key (ARK)
ark:/b7295/w9cil25778
Grouping This image is part of a group.
Imaging
Image Type
recorded image
time lapse microscopy
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
probe for lipid
BODIPY 564/570
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 813px ——
Y 695px ——
Time 300 seconds 56


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