Licensing

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Description

HVEM image of thick section of a polytene chromosome from the fat bodyof 3rd instar larvae of Drosophila melanogaster showing the organization into bands and interbands characteristic of polytene chromosomes. This image was taken with a tilt angle of -5 degrees. The accompanying grouped image, taken at +5 degrees forms a stereo pair, providing a 3D view of the chromosome.

Technical Details

Cells were dissected, fixed with formalin, stained with uranyl acetate, embedded in epoxy resin, and 0.5 micrometer sections prepared (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
Cell Type
fat body cell
Cellular Component
polytene chromosome

Biological Context

Biological Process
chromosome organization

Attribution

Name
Hans Ris

Grouping

This image is part of a group.

Imaging

Image Type
film
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
tungsten compound
Processing History
unprocessed raw data

Sample Preparation

Methods
formaldehyde fixed tissue
tissue in epoxy resin embedment
Relation To Intact Cell
microtome-sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 6600px 1.0nm
*CIL – Cell Image Library accession number. Please use this to reference an image.