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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24921*  Cite 
Description

Ancistrum is a marine ciliate that inhabits the mantle cavity of mytiliid mussels. This image shows a good view of the macronucleus, mitochondria with tubular cristae, and what are probably flask-shaped endosomes. This group of images is located in a part of the cytoplasm that especially contains these vesicles, and this image has a view at an appropriate angle to exhibit what may be dynamin or a dynamin-like protein decorating the tube of the flask shaped organelle. In addition, the macronuclear membrane is well demonstrated. TEM taken by G. Antipa in 1969 on a Hitachi HU11A operating at 75kV. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 60nm thickness.

Biological Sources
NCBI Organism Classification
uncultured Scuticociliatia
Ancistrum sp.
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
macronucleus
cytoplasm
mitochondrion
mitochondrial crista
mitochondrial envelope
peroxisome
endosome
Attribution
Names
Gregory Antipa (San Francisco State University)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24921
Archival Resource Key (ARK)
ark:/b7295/w9cil24921
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 5696px 1.72nm
Y 5532px 1.72nm


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