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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24784*  Cite 
Description

Localization of XAC (Xenopus ADF/cofilin) in Xenopus fibroblasts. Immuno-EM with XAC antibody at low magnification. High magnification view is available at CIL 24785. Nucleus and surrounding regions look bright because of high content of cellular proteins, which creates high electron density (brightness in inverted contrast). Immunogold-labeling in these central regions is very low. Image corresponds to Figure 10h from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
fibroblast
Cellular Component
lamellipodium
Xenopus ADF/cofilin
nucleus
Biological Context
Biological Process
actin filament organization
ruffle organization
Molecular Function
structural constituent of cytoskeleton
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24784
Archival Resource Key (ARK)
ark:/b7295/w9cil24784
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
optical path length gradient
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
platinum replica
primary antibody plus labeled secondary antibody
immuno gold
Processing History
unprocessed raw data
Sample Preparation
Methods
permeabilized tissue
glutaraldehyde fixed tissue
polyethylene glycol
Relation To Intact Cell
dispersed cell in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 2753px ——
Y 1878px ——