Plectin sidearms and intermediate filaments labeled with plectin immuno-gold (10 nm) in control cells. Cells with a similar label, but with actin removed by gelsolin treatment appears as CIL: 23403.
Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon.performed after cell lysis as described (Svitkina et al., 1995). Image corresponds to Fig 1d in J Cell Biol. 1996 Nov;135(4):991-1007.