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Description

Plectin sidearms and intermediate filaments labeled with plectin immuno-gold (10 nm) in control cells. Cells with a similar label, but with actin removed by gelsolin treatment appears as CIL: 23403. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon.performed after cell lysis as described (Svitkina et al., 1995). Image corresponds to Fig 1d in J Cell Biol. 1996 Nov;135(4):991-1007.

Biological Sources

NCBI Organism Classification
Rattus
(Old World rats)
Cell Type
fibroblast
Cell Line
REF-52
Cellular Component
intermediate filament
plectin
actin cytoskeleton

Attribution

Names
Tatyana M. Svitkina
Alexander B. Verkhovsky,
Gary G. Borisy
Published
J Cell Biol. 1996 Nov;135(4):991-1007
Pubmed
8922382

Grouping

This image is part of a group.

Sample Preparation

Methods
glutaraldehyde fixed tissue
tannic acid
critical_point dried specimen
Relation To Intact Cell
platinum replica

Dimensions

Spatial Axis Image Size Pixel Size
X 3466px ——
Y 3318px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.