Alternate header for print version

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:20815*  Cite 

The decorated spongiome of the CVC is composed of 50nm tubules that have a pattern of helically wound subunits attached to their cytosolic surfaces. These tubules can be up to one micrometer or more in length. Each tubule ends blindly at one end but at its opposite end it empties into a duct shared by several other tubules. This common duct empties into the smooth spongiome. TEM taken 3/13/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. Adapted with permission from the J. Cell Sci. 108:3163-3170, 1995. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13118) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuolar membrane
integral to contractile vacuolar membrane
intrinsic to contractile vacuolar membrane
Biological Context
Biological Process
contractile vacuole organization
contractile vacuole discharge
Richard Allen (University of Hawaii)
J Cell Sci 108:3163-3170, 1995
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2032px ——
Y 2466px ——